rq1 dnase stop solution (Promega)

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Promega rq1 dnase stop solution
Rq1 Dnase Stop Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rq1+dnase+stop+solution/rq1+dnase/pm40530876-263-3-7
Average 90 stars, based on 1 article reviews

rq1 dnase stop solution - by Bioz Stars, 2026-10

90/100 stars

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Incubation:

Article Title: Alternative splicing of Clock transcript mediates the response of circadian clocks to temperature changes
Article Snippet: .. After spinning down, RNA pellet was washed with 200μl 70% ethanol once, resuspended in 20μl 1X RQ1 buffer (Promega, Madison, WI), and treated with 2μl RQ1 DNase (Promega) at 37°C for 30 minutes prior to the incubation with 2μl RQ1 DNase stop solution (Promega) at 65 ̊C for 10 minutes. .. cDNA was generated from equal amount of RNA for each sample using Superscript IV (Thermo Fisher Scientific, Waltham, MA).

Article Title: Characterising the Gene Expression, Enzymatic Activity and Subcellular Localisation of Arabidopsis thaliana Metacaspase 5 ( AtMCA-IIb )
Article Snippet: Total RNA was isolated from A. thaliana seedlings using the GeneJet Plant RNA Purification Mini Kit (Thermo Fisher Scientific), following the manufacturer’s instructions. .. The purified total RNA was treated with RQ1 RNAse-free DNAse (Promega, Southampton, UK) at 37 °C for one hour and the reaction terminated with RQ1 DNAse Stop Solution by incubation at 65 °C. ..

Article Title: Distinct TCR repertoire diversity, distribution, clonality gene segment use and MHC-restriction in foetal and young adult thymus
Article Snippet: A maximum of 500 ng of RNA was mixed with 1 μL RQ1 10x Buffer (Promega, cat. no: M6101) and 1 μL RQ1 DNase (1 U/μL, Promega, cat. no: M6101) and then incubated at 37 °C for 30 minutes. .. 1 μL of RQ1 DNase Stop Solution (Promega, cat. no: M6101) was then added and the mix was incubated at 65 °C to stop the reaction. .. The RNA was then reverse transcribed by adding 1.5 μL of 10 μM TRAC2 primer, 1.5 μL of 10 μM TRBC3 primer, and 1.5 μL of 10 mM dNTPs (Promega, cat. no: U1515) and incubating at 65 °C for 5 minutes.

Article Title: Protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in Pisum sativum
Article Snippet: To ensure complete removal of genomic DNA an additional DNase treatment was performed with 0.8 U of RQ1 RNase-free DNase (Promega Corp., Madison, WI, U.S.A.) per 40 μl of RNA eluate for 15 min at 37°C. .. The DNase reaction was terminated by addition of RQ1 DNase Stop Solution (Promega Corp.) and subsequent incubation for 10 min at 65°C. .. • Measure RNA concentrations (we used a Nanodrop ND-1000 Spectrophotometer (Saveen 1 Werner, Malmö, Sweden)).

Concentration Assay:

Article Title: Quorum sensing mediates morphology and motility transitions in the model archaeon Haloferax volcanii .
Article Snippet: .. Seventeen microliters of RQ1 DNase Stop Solution (Promega) was added to the DNase reaction, and 1 μL of 200 mM AEBSF (final concentration 1 nM) was added to the protease reaction. ..

Purification:

other:

Article Title: The architecture of Cidec-mediated interfaces between lipid droplets.
Article Snippet: 400 ng of RNAwas treated with 1 unit of RQ1 RNase-Free DNase (Promega, M610A) at 37 C for 30min and was inactivated by RQ1DNase Stop Solution at 65 C for 10min.

Centrifugation:

Article Title: Iron-Fur complex suppresses the expression of components of the cyclo-(Phe-Pro)-signaling regulatory pathway in Vibrio vulnificus
Article Snippet: After incubation, 0.01 unit of DNase I (Promega, Madison, WI) was added, and the reaction mixture was incubated at 37°C for 1 min. .. The reaction was terminated by the addition of 5 μL of RQ1 DNase stop solution (Promega, Madison, WI), and inactivated at 65°C for 10 min. To precipitate the samples, 55 μL phenol-chloroform was added, and the mixture was precipitated at room temperature for 5 min. After centrifugation at 11,323 × g at 4°C for 5 min sodium acetate (NaCOOH, pH 5.2) was added to 50 μL of supernatant. ..

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